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Fig. 3: MTG-catalyzed conjugation of LinkCFQ to substrate proteins with pI values of 4.78–8.34 at different pH values (pH 7, 8, and 9). | Communications Materials
Fig. 3: MTG-catalyzed conjugation of LinkCFQ to substrate proteins with pI values of 4.78–8.34 at different pH values (pH 7, 8, and 9).
The reaction was evaluated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis, followed by staining with Coomassie brilliant blue (Supplementary Fig. 4). Reactivity (%) was defined and calculated using Eq. 3. All error bars represent standard deviations. *P < 0.05.