Fig. 3: MTG-catalyzed conjugation of LinkCFQ to substrate proteins with pI values of 4.78–8.34 at different pH values (pH 7, 8, and 9). | Communications Materials

Fig. 3: MTG-catalyzed conjugation of LinkCFQ to substrate proteins with pI values of 4.78–8.34 at different pH values (pH 7, 8, and 9).

From: Extracellular matrix-inspired hydrogel of hyaluronan and gelatin crosslinked via a Link module with a transglutaminase reactive sequence

Fig. 3

The reaction was evaluated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis, followed by staining with Coomassie brilliant blue (Supplementary Fig. 4). Reactivity (%) was defined and calculated using Eq. 3. All error bars represent standard deviations. *P < 0.05.

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