Table 2 Characteristics of model proteins used for evaluating the effect of pI value on the MTG-catalyzed conjugation reaction.

From: Extracellular matrix-inspired hydrogel of hyaluronan and gelatin crosslinked via a Link module with a transglutaminase reactive sequence

Protein name

pI

MW

Lys

Gln

Total (amino acid)

EGF

4.78

6222

2

1

53

Insulin

5.22

11981

2

7

110

β-casein

5.28

44672

25

22

430

HSA

5.60

66462

59

20

583

Fibrinogen

6.36

159632

100

67

1434

Transferrin

6.70

75195

58

17

679

Myoglobin

7.36

16951

19

6

153

Lactoferrin

8.34

75982

46

26

691

  1. pI value was calculated via Expasy using the ProtParam tool (https://web.expasy.org/protparam/).
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