Fig. 5: Inhibitor combinations reduce renal CaOx deposition and injury in a mouse model of renal CaOx crystallization. | Communications Materials

Fig. 5: Inhibitor combinations reduce renal CaOx deposition and injury in a mouse model of renal CaOx crystallization.

From: Boosting inhibition performance of natural polyphenols for the prevention of calcium oxalate kidney stones through synergistic cooperativity

Fig. 5

C57BL/5 mice received an intraperitoneal injection of glyoxylate to induce renal CaOx crystallization and were concurrently treated with indicated concentrations of CA and CA/polyphenol pairs for 6 constitutive days. a Morphologic distribution of renal CaOx crystals using polarized-light optical microphotography (scale bar: 50 μm). b Representative images of Von-Kossa staining of kidney sections (scale bar: 50 μm). c Representative images of stained kidney sections with periodic acid–Schiff stain and highlighted areas of tubular injury (scale bar: 50 μm). d, e Quantified CaOx crystal deposition (d) and scored kidney injury (e) in kidney sections. f, g Plasma blood urea nitrogen (BUN, f), and serum creatinine concentration (g) were measured on day 7. The raw data was plotted on the upper axes with line breaks denoting the mean value of each group and lines indicating the standard deviation. The mean differences for six comparisons against the shared positive control, Gly, were plotted as bootstrap distributions on the bottom axes. Each mean difference is depicted as a dot and each 95% CI is indicated by the ends of the vertical error bars.

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