Fig. 2: Strategies used in rational engineering of PET degrading enzymes.
Strategies are selected from those discussed in this Review for engineering. a PET hydrolases and b MHET hydrolases. Modeled structures are shown only to be representative, with lines indicating approximate locations on the enzyme scaffold (or analogous location mapped from homologous scaffold) where mutation(s) would be made. The modeled PET hydrolase is LCC-ICCG (PDB: 6THT29) and the modeled MHET hydrolase is IsMHETase (PDB: 6QZ473). Color(s) of boxes and text indicate the design goal the mutation(s) aim to achieve; two colors indicate the mutation(s) may affect multiple properties.
