Fig. 6: Overview of selected high-throughput screening methods. | Communications Materials

Fig. 6: Overview of selected high-throughput screening methods.

From: Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics

Fig. 6

a Microtiter plate-based assays. The overview is based on the screen by Shi, et al., to engineer DepoPETase122, but similar workflows could be applied to using absorbance (change in turbidity)116, color (change in pH)117, or UV absorbance (aromatic product release)118,119 as readouts. b UHPLC screening52. c FADS129. Note: the authors did not use the screen to engineer enzymes, but the screen could be adapted to screen enzymes using directed evolution similarly to the other methods. A similar workflow could be used with a TPA biosensor132, where the cells would need to be encapsulated for the reaction and the TPA would need to be imported. d Yeast display coupled with FACS133. e Coupled BHET and split-GFP screens on agar plates35,53,135.

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