Fig. 2: Overview of the metabolites detected across the three analytical platforms employed and their respective ordinations.
From: Adding labile carbon to peatland soils triggers deep carbon breakdown
Panel (A) displays a merged histogram of the exact masses (amu) of metabolites detected across Nuclear Magnetic Resonance (1H NMR), Gas Chromatography-Mass Spectrometry (GC MS), and Liquid Chromatography-Tandem Mass Spectrometry (LC MS/MS) analytical platforms. The total number of metabolites from each method used in subsequent analyses is indicated in parentheses in the legend. Panel (B) depicts the Principal Coordinate Analysis (PCoA) ordinations generated using Bray-Curtis distances for each analytical method. The variation explained by the first two coordinate axes is mentioned under each plot. Samples are colored based on sampling depth and shaped according to timepoints, as shown in the legend below. The statistical significance of sample separation based on soil depth (surface vs. deep), treatment effect (no glucose vs. glucose), and time (days 7–70) was assessed using permutational multivariate analysis of variance (PERMANOVA) through the adonis2 function in R112. Only the significant components and interactions are presented next to each ordination plot, as per the legend. Individual PCoA ordinations for each depth are provided in Supplementary Fig. 2.
