Extended Data Fig. 7: Effects of conditioned media from SIRT3-overexpressing human and mouse lung epithelial cells on FoxO3a levels in human lung fibroblasts. | Nature Aging

Extended Data Fig. 7: Effects of conditioned media from SIRT3-overexpressing human and mouse lung epithelial cells on FoxO3a levels in human lung fibroblasts.

From: Restoration of SIRT3 gene expression by airway delivery resolves age-associated persistent lung fibrosis in mice

Extended Data Fig. 7

(a) Schematic of the experimental design. (b) Left panel, representative western blot showing overexpression of SIRT3 in A549 cells. Right panel, western blot showing levels of FoxO3a in human fibroblasts incubated with the conditioned media from SIRT3-overexpressing A549 cells. Cond. Med. = conditioned media (c) Left panel, western blot showing overexpression of SIRT3 in L2 mouse epithelial cells. Right panel, representative western blot showing levels of FoxO3a in IMR-90 fibroblasts incubated with conditioned media from SIRT3-overexpressing L2 cells at 48 hours. Cond. Med. = conditioned media.

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