Extended Data Fig. 9: FoxO3a levels in young and aged mouse fibroblasts co-cultured with SIRT3 overexpressing L2 cells.

(a) Schematic of the experiment design; FB = fibroblasts. (b) Representative western blots indicate SIRT3 levels in whole cell lysates of L2 cells (b), FoxO3a levels in the cytoplasm (c), and in nuclear fractions (d) from young and old mouse fibroblasts. Fibroblasts were co-cultured with L2 cells that overexpress SIRT3 or control plasmid.