Extended Data Fig. 1: Additional data related to food odor suppression of DR longevity without increasing food ingestion. | Nature Aging

Extended Data Fig. 1: Additional data related to food odor suppression of DR longevity without increasing food ingestion.

From: Olfactory perception of food abundance regulates dietary restriction-mediated longevity via a brain-to-gut signal

Extended Data Fig. 1

a, b, Food odors do not change the pumping rate of worms under DR. Pumping rate was counted at 24 hr (a), and 96 hr (b), after worms were transferred to the DR assaying plates. 2 × DR: twice amount of bacterial food, which stimulated the pumping rate. n = 30 (DR), 30 (DR + odor), 16 (2xDR) and 16 (2xDR + odor) biologically independent animals in (a). n = 30 (DR), 30 (DR + odor), 15 (2xDR) and 14 (2xDR + odor) biologically independent animals in (b). Data are presented as mean ± s.e.m. P values were calculated with one-way ANOVA with Bonfferroni’s test. See Fig. 1c for data of the pumping rate counted at 1 hr post-transfer. c, Feeding worms with twice amount of bacteria food (2x DR) does not affect DR longevity, but food odors can still suppress the lifespan of these worms.

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