Extended Data Fig. 4: miR-31 up-regulation in skin epithelium induces HFSC transepidermal differentiation and aging-like phenotypes.
From: A stress-induced miR-31–CLOCK–ERK pathway is a key driver and therapeutic target for skin aging
a, IF staining images of backskin sections of no Wax/post Wax 48 hr WT/DTG littermates. Scale bars: 50μm. b, Backskin surface images of Tam-labeled WT/DTG Sox9-CreER;mTmG littermates after Dox induction and Wax. The mice were waxed at P50 ( + Dox and Tam since P48) and photographed at day 3 post waxing. Scale bars: 2 mm. c, IF staining images of backskin sections of post Wax day0 and day 3 WT/DTG littermates. They were waxed at P50 ( + Dox since P48). Scale bars: 50μm. All above error bars represent standard errors. d, Merged white-field/GFP/tdTomato images of day 4 exoplant cultures of backskin samples from the above Fig. 2j mice at P50 before Wax, and quantification (n = 6 exoplants separated from 3 mice) of GFP + % among tdTomato+ cells in the MK sheets. Scale bars: 100 μm. For all relevant figures, data are represented as mean ± SEM. P values were calculated with two tail t-test.
