Extended Data Fig. 5: Conditional miR-31 ablation inhibits stress-induced HFSC transepidermal differentiation and premature aging.
From: A stress-induced miR-31–CLOCK–ERK pathway is a key driver and therapeutic target for skin aging
a, Fluorescent images of backskin sections of P50 Tam-labeled Sox9-CreER;mTmG WT/cKO littermates at day 2 post Tam injection. Scale bars: 50μm. GFP is in green, tdTomato is in red. b, IF staining of backskin sections of P50 Tam-labeled Sox9-CreER;mTmG WT littermates at day 2 post Tam injection. Arrow head indicate c-Kit+ melonocytes. Scale bar: 50μm. c, Representative FACS sorting profiles of CD49f+GFP+(G+) and CD49f+GFP- (G-) cells from backskin epithelium of Tam-labeled Sox9-CreER;mTmG WT/cKO littermates at day 2 post Tam injection. d, DNA gel electrophoresis of genomic DNA PCR products of Mir31 surrounding regions. G-/G+: FACS sorted CD49f + GFP + and CD49f + GFP- cells described in B. Left labels: DNA size marker in bp, Right labels: expected PCR band positions and bp size of the indicated alleles. e, Upper panel: schematic of mouse genomic region around Mir31 gene and positions of the PCR primers used above (arrows). No other coding genes are present in vicinity of Mir31. Lower panel: DNA sequencing results of the indicated genomic region in the FACS sorted CD49f + GFP + backskin epithelium cells from Tam-labeled Sox9-CreER;mTmG WT/cKO littermates. f, Backskin photographs of WT/cKO littermates at indicated ages. The mice were Tam injected at P19. Scale bars: 1 cm. g, Backskin photographs of a pair of WT/cKO littermates at their day 0 (d0, P50), day 8 (d8) and day 10 (d10) post Waxing. The mice were Tam injected 2 days before waxing at P48. Scale bars, 1 cm. h, IF staining of the above g mice backskin sections at day 14 post wax. Scale bars: 50μm.
