Extended Data Fig. 2: Comparison of age-related changes in each age group.
From: Healthy aging and muscle function are positively associated with NAD+ abundance in humans
(a) Volcano plots of fold change (x axis, log2 scale) versus p value (y axis, -log10 scale) for trained older adults (top; n = 17), and physically impaired older adults (bottom; n = 6) compared to young individuals (n = 12), illustrating significantly depleted (blue) or accumulated (red) metabolites with age. Line indicates significance (p < 0.05). Significance was determined using an empirical Bayes moderated t test (two-sided, p values adjusted for multiple comparisons between groups). (b) Venn diagram of the overlap of significantly higher or lower abundances of metabolites in each aged group (trained older adults, older adults with normal physical activity levels, physically impaired older adults) compared to young individuals (p < 0.05). Source data: Statistical_Source_Data.csv, all exact p values for comparison between groups are listed therein.
