Extended Data Fig. 5: A time course analysis showing that CUX1 knockdown inhibits the p16INK4a-dependent and bleomycin-induced cellular senescence in human ECs.
From: Post-GWAS functional analysis identifies CUX1 as a regulator of p16INK4a and cellular senescence
A. Western blot analysis showing that knockdown of CUX1 by shRNA inhibits the upregulated p16INK4a expression induced by 0.5 µg/ml bleomycin treatment over a 48 hrs time course. Data for the Western blot analysis represents three biologically independent experiments (n=3). B. γ-H2AX staining demonstrating EC senescence induced by bleomycin treatment can be inhibited by downregulation of CUX1 over the same time course. C. Quantitative plots showing the γ-H2AX foci/cells (%) in γ-H2AX staining. Data for γ-H2AX staining represents three biologically independent experiments (n=3).
