Fig. 7: Demonstration that CUX1 regulates replicative senescence independently of p53 expression.
From: Post-GWAS functional analysis identifies CUX1 as a regulator of p16INK4a and cellular senescence
a, Immunoblot analysis showing passage-dependent induction of p53 expression in p10 compared to p5 ECs. qPCR analysis showing that induction of p53 was not at the transcriptional level. b, Immunoblot (left) and qPCR analysis (right) showing no significant change in p53 expression following shRNA-mediated CUX1 knockdown in p10 ECs. c,d, Immunoblot and qPCR analysis showing no significant change in CUX1 expression (d) after p53 siRNA-mediated knockdown (c) in human ECs. e,f, SA-β-gal (e) and γ-H2AX (f) staining showing no significant change in EC senescence by comparison of scrambled siRNA control ECs with p53 siRNA knockdown ECs. Data for immunoblot analysis represent n = 3 biologically independent experiments; data for qPCR analysis represent n = 3 biologically independent samples, each performed in duplicate. Data for SA-β-gal and γ-H2AX staining represent n = 3 biologically independent experiments. P values were calculated using two-tailed Student’s t-test, and all data are presented as mean ± s.e.).
