Extended Data Fig. 1: Immunohistochemical characterization of C1q experimental cohort.
(a) Longitudinal volumetric T2 weighted MRI quantification of whole brain volume changes in WT and C3KO mice at 6 and 9 months (normalized to 3 months). (b) Example C1q immunofluorescence images from hemibrains of each genotype used in the study. (c) Quantification of C1q immunofluorescence in the whole brain of 9-month-old mice. (d) Representative images showing AT8 (pTau), Iba1, GFAP in hemibrains and Amino Cupric Silver staining in the hippocampus. (e) Quantification of pTau, Iba1, GFAP and Amino cupric silver positive area in whole brains. (f) Quantification of the same markers as in E) with analysis restricted to the hippocampus. (g) Representative images showing NeuN staining in each genotype with example ROIs for the CA1, CA3, and dentate gyrus (DG), subfields are illustrated on the first image. (h) Percentage of NeuN+ area in hippocampal CA1, CA3 and DG subregion across genotypes. Each dot shows average data from one mouse. 13–14 mice/genotype were used for volumetric MRI experiment in a), 8–10 mice/genotype were analyzed by immunohistochemistry in c-h). One-way ANOVA with Tukey multiple comparison test was used. All data are presented as mean ± SEM.
