Extended Data Fig. 1: Controls and correlations between spike-specific responses and Charlson Index, days post vaccination or age.
From: Signs of immunosenescence correlate with poor outcome of mRNA COVID-19 vaccination in older adults

(a) Correlation between frequency of S-specific IFN-γ response and Charlson co-morbidities index (r = −0.417, P = 0.0005) or (b) days after the first vaccination (not significant). (c) Influenza (light blue, n = 22; red n = 24; grey n = 16) and (d) CD3 (light blue, n = 22; red n = 23; grey n = 18) specific response in vaccinated subjects of various ages. (e) S-specific IFN-γ response in SARS-CoV-2 exposed subjects (n = 46) and correlation with age (not significant). (f) Frequency of IFN-γ SFCs, in response to Spike (S; orange), Nucleocapsid (Nu; green), and Membrane (M; blue) proteins in SARS-CoV-2 exposed subjects (n = 49). The numbers in the below the graph represent the percentage of responders (R) and non-responders (NR) for each antigen. The cut-off value for a positive response is defined as described in the Methods section. Each dot represents a single study subject and horizontal lines indicate medians. The red line represents linear regression and two-tailed Spearman’s test was used to the test the significance (r and P values). P values were determined by two-tailed Kruskal-Wallis test with Dunn’s multiple comparison correction; ** P < 0.01; ns: not significant; yr: years.