Extended Data Fig. 2: Gating strategy and examples of flow cytometry measurements.
From: Signs of immunosenescence correlate with poor outcome of mRNA COVID-19 vaccination in older adults

(a) Gating strategy to identify CD4 + and CD8 + T cell phenotype and cytokines production. (b) Representative flow cytometry plots identifying CD4 + (left panel) and CD8 + (right panel) producing IFN-γ, IL-2 and TNF-α in response to S peptides (bottom panel) and DMSO negative control (upper panel). Cytokines (IFN-γ, IL-2 and TNF-α) were analyzed on non-naïve (memory) T cells.