Extended Data Fig. 5: Evaluation of hematopoietic stem and progenitor-cell population representation from young and aged Phf6 wild-type and knockout mice.
From: Epigenetic reversal of hematopoietic stem cell aging in Phf6-knockout mice
a, Representative FACS plots of the total stem and progenitor subset (LSK) and committed myeloid progenitor population (MyP) frequency in the bone marrow of 8-week-old Phf6 wild-type (Phf6+/Y) and hematopoietic-specific knockout mice (Phf6−/Y). b, Quantification of absolute cell numbers of LSK and MyP populations, as in a. Total cell numbers are shown for n = 5 animals per group, per genotype. c, Representative FACS plots of LSK and MyP population frequency in the bone marrow of 24-month-old Phf6 wild-type (Phf6+/Y) and knockout mice (Phf6 −/Y). d, Quantification of absolute cell numbers of LSK and MyP populations, as in c. Total cell numbers are shown for n = 8 and n = 9 animals per genotype, respectively. e. Quantification of absolute cell number/animal of LT-HSC, ST-HSC, MPP2, MPP3 and MPP4 populations in the bone marrow of 24-month-old Phf6 wild-type (Phf6+/Y) and knockout mice (Phf6−/Y). Total cell numbers are shown for n = 8 and n = 9 animals per genotype, respectively. f, Gating strategy for phenotypic identification and quantitation of LT-HSC, ST-HSC, MPP2, MPP3 and MPP4 populations by FACS. g, Gating strategy for phenotypic identification and quantitation of CLP progenitor cells by FACS. h-j, Complete blood count analysis of white blood cells (WBCs), neutrophils (NE) and lymphocytes (LY). k-n, Complete blood count analysis of hematocrit (HCT), red blood cells (RBC), hemoglobin (Hb) and mean corpuscular volume (MCV). o, Complete blood count analysis of platelets (PLT). Peripheral blood counts were scored in 4-month-old wild-type (Phf6+/Y), 4-month-old knockout (Phf6−/Y), 21-month-old wild-type (Phf6+/Y) and 21-month-old knockout (Phf6−/Y) animals (n = 4 mice per group). Graphs represent mean ± SD and p-values were assessed using two-tailed unpaired Student’s t-test.
