Extended Data Fig. 7: LT-HSC differentiation potential and surface expression analysis of age-associated markers in HSCs recovered after secondary transplantation of LT-HSCs from old Phf6 wild-type and knockout donor mice.
From: Epigenetic reversal of hematopoietic stem cell aging in Phf6-knockout mice
a, Representative contour plots of CD150 and CD48 surface expression and quantitation gates for LT-HSC, ST-HSC, MPP2 and MPP3 stem and progenitor cell subsets within the bone-marrow LSK fraction of recipient mice of serially transplanted aged Phf6 wild-type and knockout LT-HSCs. b, Representative pseudo-color density plots depicting the frequency of donor-derived (CD45.2+) and residual host-derived (CD45.1+) cells in peripheral blood of mice serially transplanted with aged Phf6 wild-type and knockout LT-HSCs. c, Representative histograms indicating CD150, CD49b and CD41 expression on donor-derived LT-HSCs recovered from mice transplanted with aged Phf6 wild-type and knockout LT-HSCs. Representative data relates to Fig. 3.
