Extended Data Fig. 3: ATAC-Seq – additional information.
a Schematic distribution of the ATAC peaks on the four main Drosophila chromosomes. Additional peaks were detected on contigs and are not shown. b Violin plots showing the size of all ATAC peaks detected in the gut and fat body, and in the significantly differentially accessible peaks in the fat body. c Distribution of the distance of ATAC peaks from a transcriptional start site (TSS). Proportion of peaks in each distance category are presented from 5’ to 3’ relative to the TSS. d ATAC-qPCR of the levels of sequences near the MED1 locus where an ATAC-Seq peak opened by dfoxo-switch was detected, near Prosap where a peak unaltered by dfoxo-switch was detected, and Sox21b region which contained no peak in ATAC-Seq. Boxplots – quantiles; whiskers – extremes; overlay – individual data points. N = 3 biologically independent samples; effect of dfoxo-switch: MED1 p = 0.00024, Prosap p = 0.59, Sox21b p = 0.76, pairwise comparisons with two-sided unpaired t-tests with pooled SD. e ATAC-qPCR signal intensity for regions near the Xbp1 locus within a peak detected by ATAC-Seq (3’end of the gene) or two regions within the promoter of Xbp1. The levels were normalized to Prosap. Boxplots – quantiles; whiskers – extremes; overlay – individual data points. N = 3 biologically independent samples, effect of region p = 0.0011, effect of dfoxo-switch p > 0.05, mixed effects LM.
