Extended Data Fig. 4: ANC-1 modulates nucleolar size.
From: Nucleophagy delays aging and preserves germline immortality
a, Quantification of FIB-1::GFP puncta area from Fig. 4a. The box plot represents the 25th–75th percentiles, the line depicts the median and the whiskers show the min–max values. ****P<0.0001 using one-way ANOVA; n ≥ 24 puncta. b, Western blot analysis and quantification of relative endogenous FIB-1 levels of day 3 adult WT worms and anc-1(e1753) mutants. Mean ± s.d. of three biological replicates. **P<0.01 using unpaired t-test. c, Life span analysis of EAT-2-deficient worms subjected to RNAi-mediated silencing of anc-1. d, Life span analysis of long-lived DAF-2- and FIB-1-deficient worms subjected to RNAi-mediated silencing of anc-1 and lgg-1. n ≥ 88 worms e, Confocal microscopy imaging of FIB-1::GFP;mKate::ANC-1-expressing day 2 worms. Enlarged images of the indicated numbered squares are shown on the right panel. Scale bar, 20μm.
