Extended Data Fig. 3: ANC-1/Nesprin 2 regulates LC3B abundance and localisation.
From: Nucleophagy delays aging and preserves germline immortality
a, Epifluorescence microscopy imaging of head, intestine and seam cells of GFP::LGG-1 transgenic worms subjected to RNAi-mediated silencing of lgg-1, anc-1, bec-1, rab-7, anc-1;bec-1 and anc-1;rab-7. Scale bars, 20μm. b, Quantification of GFP::LGG-1 pixel intensity in head from a. The box plot represents the 25th–75th percentiles, the line depicts the median and the whiskers show the min–max values. ***P<0.001, ****P<0.0001 using one-way ANOVA; n ≥ 20 worm head regions. c, d, e, f, Quantification of LC3 puncta size, nuclear LC3B pixel intensity, perinuclear LC3B puncta per cell and perinuclear LC3B puncta size in control and BafA1-treated WT and Nesprin 2-/- E14.5 MEFs from Fig. 2e. The box plots represent the 25th–75th percentiles, the lines depict the median and the whiskers show the min–max values. ****P<0.0001 using two-way ANOVA; n ≥ 44 puncta, n ≥ 31 nuclei, n ≥ 20 cells and n ≥ 8 puncta respectively.
