Extended Data Fig. 1: GDF11 treatment does not affect anxiety-like state, general well-being or physical performance.
(a–c) Behavioral tests related to anxiety: (a) Measurement of the distance moved in the Light Box, during the Light/Dark Box test (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 12 mice; F (2, 31) = 12.8), (b) Measurement of the time spent in the Light Box, during the Light/Dark Box test (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 12 mice; F (2, 31) = 8.5), (c) Measurement of the time spent in the open arms, during the Elevated Plus Maze test (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 12 mice; F (2, 31) = 1.0). (d–f) Behavioral tests related to general well-being: (d) Measurement of the height of the nest (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 12 mice; F (2, 31) = 0.8), (e) Scoring of the nest quality based on five criteria (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 12 mice; F (2, 31) = 1.2), (f) measurement of the burrowing index (percentage of weight of pellets removed from the burrow divided by total pellet weight), during the Burrowing test (nYoung = 6 mice, nAged = 9 mice, nAged+GDF11 = 9 mice; F (2, 21) = 0.5). (g–j) Behavioral tests related to physical performance: (g) measurement of the distance moved during the Open Field test (nYoung = 11 mice, nAged = 11 mice, nAged+GDF11 = 12 mice; F (2, 31) = 2.7), (h) measurement of the time mice spent hanging on the wire during the Hanging Wire test (nYoung = 10 mice, nAged = 10 mice, nAged+GDF11 = 10 mice; F (2, 27) = 3.6), (i) measurement of the hind paw and fore paw stride length during the Gait test (nYoung = 10 mice, nAged = 13 mice, nAged+GDF11 = 11 mice; F (2, 62) = 0.03), (j) measurement of the hind base and front base length during the Gait test (nYoung = 10 mice, nAged = 13 mice, nAged+GDF11 = 11 mice; F (2, 62) = 7.4). One-way and two-way ANOVA and Tukey’s post hoc test for multiple comparisons; F (DFn, DFd) value presented for each ANOVA statistical analysis; P values < 0.05 are represented on the graph; mean ± s.e.m.
