Extended Data Fig. 3: Loss of Altre in CD4⁺ T cells does not affect the development and homeostasis of CD4⁺ T or Treg cells at a young age.

a, The two lox sites were inserted into the third and fourth exons of the Altre locus by embryonic stem cell recombination technology. b, Representative flow cytometry gating strategy for thymocyte. c, Representative flow cytometry gating strategy for CD4⁺ T cell and CD8⁺ T cell in spleen, lymph nodes, liver, VAT (visceral adipose tissue), and colon. d, Representative flow cytometry plots of CD4⁺ and CD8⁺ subsets in the thymus (up panel) and spleen (down panel) from Altre^f/f and Altre^f/f Cd4^Cre mice (2 months old; n = 3). e, Statistical analysis of the percentage of different T cell subsets in the thymus and spleen from young Altre^f/f and Altre^f/f Cd4^Cre mice as in d. Two-tailed t-test. f, The relative level of Altre and its neighboring genes Igf1r, Pgpep1l, and Fam169b were analyzed by qRT-PCR in Treg cells from Altre^f/f and Altre^f/f Cd4^Cre mice (n = 4). Two-tailed t-test. g, Statistical analysis of the percentage of CD45⁺CD4⁺CD25⁺Foxp3⁺ Treg cells in the thymus, spleen, liver, and VAT from Altre^f/f and Altre^f/f Cd4^Cre mice (2 months old; Altre^f/f, n = 5; Altre^f/f Cd4^Cre, n = 6). Two-tailed t-test. h, The relative level of Il10, Pdcd1, Ctla4, and Il2ra were analyzed by qRT-PCR in Treg cells from young Altre^f/f and Altre^f/f Cd4^Cre mice (2 months old; n = 4). Two-tailed t-test. Data are mean ± s.d. NS, non-significant.

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