Extended Data Fig. 9: The inflammasome activity of primary microglia, primary astrocytes cell, microglial cells line BV2, and astrocyte cell line MA-c after treated with IAA in vitro (n = 6).
From: Chiral nanoparticle-remodeled gut microbiota alleviates neurodegeneration via the gut–brain axis
(A) levels of NLRP3 in microglia cell line BV2 and astrocyte cell line MA-c were determined confocally. Scale bars, 20 μm. (B) Western blot analysis of microglia cell line BV2 and astrocyte cell line MA-c for NLRP3, pro-caspase 1, caspase 1, ASC, pro-IL18, IL18, pro-IL1β, IL1β, NF-κB and β-actin, respectively. The expression levels of NLRP3, caspase-1, IL1β and IL18 in (C) microglial cell line BV2 and (D) astrocyte cell line MA-c were detected by RT-qPCR. Contents of IL18 and IL1β in (E) primary microglia and (F) primary astrocytes cell were determined by ELISA. Contents of IL18 and IL1β in (G) microglia cell line BV2 and (H) astrocyte cell line MA-c were determined by ELISA. CH-223191, the AHR inhibitor. Each ‘n’ represents an independent biological sample. One dot represents one sample. Data are represented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. One-way (C-H) ANOVA followed by Tukey’s multiple comparisons test.
