Extended Data Fig. 5: Mitophagy inducer urolithin A modulates DAF-12/FXR activity.
a, Human FXR activity was measured post-treatment with GW4064 and MIC. Assay was independently repeated four times. Graph show mean ± s.d. GW4064 (p < 0.0001), (P-value via one-way ANOVA with Tukey’s multiple comparisons test). b, left: UA and MIC structure is shown with benzocoumarin rings depicted in grey. Right: Human FXR activity was measured post-treatment with GW4064 and (+/− UA). Assay was independently repeated four times. Graph show mean ± s.d. GW4064 vs ctrl (p < 0.0001) and GW4064-UA50 (p = 0.0012). (p-value via two-way ANOVA with Tukey’s multiple comparisons test). c, DAF-12 activity post-dafachronic acid treatment, alone or with UA, was quantified by one-hybrid reporter assay. Graph show mean ± s.d. Assay was independently repeated three times. DMSO + Ligand (p = 0.0005), Ligand+ UA30 (p = 0.0076) Ligand +UA50 (p = 0.0069), (P-value via one-way ANOVA with Tukey’s multiple comparisons test).
