Extended Data Fig. 4: Lifespan extending effect of MIC.
a, Survival curve of WT(N2) animals, treatment (median days); N2-DMSO (22) and N2-MIC (29), ****p < 0.0001 (p-value via Mantel-Cox long-rank test). MIC exposure from days 1–12 (15 °C) of adulthood. Detailed statistical information for all lifespans is presented in Supplementary Table 1. b, Survival curve of WT(N2) animals, treatment (median days); N2-DMSO (17) and N2-MIC (17), MIC exposure (15 °C) during development only (egg to young adult). c, Left: Survival curve of WT(N2) and hlh-30(tm1978) animals on dafadine A (DFA) post-development, N2-DMSO (17) and N2-DFA (19); p = 0.0021, hlh-30-DMSO (15) and hlh-30-DFA (12); p < 0.0001, (p-value via Mantel-Cox long-rank test). Assay was independently repeated three times. Right: Survival of day-2 WT(N2) and hlh-30(tm1978), 3 h post-FCCP (20 °C). Assay was independently repeated two times, ≥30 animals/repeat. WT-DMSO vs WT-DFA 20 μM; (p = 0.0113), hlh-30-DMSO vs WT-DFA 40 μM; (p = 0.0116), (p-values via two-way ANOVA with Sidak’s multiple comparisons test). d, Survival curve of pdr-1(gk448) animals, pdr-1-DMSO (15) and pdr-1-MIC (17), (p = 0.0009) (p-value via Mantel-Cox long-rank test). Assay was independently repeated two times. MIC exposure from days 1–7 (20 °C) of adulthood. e, Median lifespan shown for conditions/genotypes post treatment. N2 (D-20 °C) indicates MIC treatment only during development. ‘Life-long’ means exposure throughout the life starting from day-of adulthood. Each dot depicts median lifespan from a plate of ~50 animals. Lifespan change is averaged across trials. DMSO vs MIC for N2(15 °C)-life-long; (p = 0.0149), N2(20 °C)-life-long; (p = 0.0400), N2(15 °C)-day1–15; (p < 0.0001), N2(20 °C)-day1–7; (<0.0001) and mev-1(20 °C)-day1–7; (p = 0.0020), (p-value via two-tailed unpaired t-test). Detailed statistical information for all lifespans is presented in Supplementary Table 1.
