Extended Data Fig. 4: Downregulation of age-dependent gene upregulates NSC quiescence markers. | Nature Aging

Extended Data Fig. 4: Downregulation of age-dependent gene upregulates NSC quiescence markers.

From: Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Extended Data Fig. 4

a, Heatmap showing scRNA-seq differential expression analysis of the age-dependent genes identified in bulk RNA-seq in each NSPC cluster. Data are the average log2 fold change of expression. b, Heatmap showing bulk RNA-seq differential expression analysis of DE genes detected in early type B1a cells as in Fig. 1j. Fold change of RNA level (Aged_FPKM vs. Young_FPKM) and p-value were plotted. c, d, Quantitative real-time PCR analysis of relative expression (siRNA group vs. siControl group) of Aldoc and Atp1a2 in young NSPCs 48 hours after siRNA transfection. n = 3 independent assays. Error bars are mean ± SD. Unpaired two-sided Student’s t-test.

Source data

Back to article page