Extended Data Fig. 2: Identification of NSPC activation states and lineage specificity through single-cell transcriptome. | Nature Aging

Extended Data Fig. 2: Identification of NSPC activation states and lineage specificity through single-cell transcriptome.

From: Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Extended Data Fig. 2

a, Heatmap of top 10 differentially expressed marker genes in 4 clusters of NSPCs. Analysis was performed using merged scRNA-seq datasets of young and aged NSPCs. b, Ridgeline plots of merged scRNA-seq datasets of young and aged NSPCs, showing expression changes of activation markers from early type B1a cells to late type C cells. c, Violin plots showing the similar expression levels of NSPC lineage markers among 4 clusters between young and aged NSPCs. d, UMAP plots of NSPC lineage markers in young and aged NSPCs. e, Single-cell pseudotime analysis using Monocle3 shows dynamic transition of NSPC activation states along the trajectory. NSPCs with larger pseudotime values exhibit higher activity in proliferation. f, Gene ontology analysis of the DE markers of early type B1a cells. The gene number of each GO category is showed on the right side of the bar chart.

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