Extended Data Fig. 6: TXN knockdown does not affect cGAS localization or activity.
a–d, Expression of the indicated proteins in IMR90 cells induced to undergo senescence by oncogenic RAS expressing shControl or shTXN (a). The indicated cells were stained for γH2AX and cGAS. DAPI counter staining was used to visualized nuclei. Arrows point to examples of cGAS positive CCFs (b), which was quantified (c). Further, 2’3’-cGAMP levels in the indicated cells were quantified (d). Scale bar = 10 μm. Data represent mean ± s.e.m. n = 3 biologically independent experiments.
