Extended Data Fig. 9: Tri-1 and auranofin do not affect p16 and p53 signatures in aged mouse ovaries.

a, Heatmap of the SASP genes that were significantly upregulated in ovaries from aged mice (22 months) compared with young mice (4 months) (n = 10 biologically independent mice in young group, n = 9 biologically independent mice in aged group). b, Heatmap of the SASP genes that were significantly suppressed by Tri-1 treatment in aged mouse ovaries (n = 4 biologically independent mice per group). c-e, Ingenuity Pathway Analysis of the 1920 genes that were significantly different in aged vs young mice ovaries. Common gene expression changes induced by Tri-1 and auranofin treatments showed expected common inhibition of GSR and TXNRD1 regulators (c). Transcription factors with altered activity were listed with p53 and p16 among them (d). P values were calculated by a Fisher Exact Test estimated by Ingenuity Pathway Analysis Software. Both these two age-associated signatures were not affected with either Tri-1 or auranofin treatment in the aged mice (e). P values were calculated by hypergeometrical test.