Extended Data Fig. 10: Suppression of SASP by Tri-1 treatment in aged mouse ovary.
a, Expression of the indicated SASP genes in ovaries from young (4 months) or aged (22 months) mice treated with Tri-1 or vehicle control was determined by RT-qPCR. n = 4 biologically independent mice per group. b,c, Immunoblot of the indicated proteins in the ovary tissues harvested from aged mice (22 months) with or without Tri-1 or auranofin treatments (b). The intensity of the indicated proteins was quantified by NIH ImageJ software and normalized against a loading control β-actin expression (c). n = 5 biologically independent mice per group. d, Expression of p16 in ovaries from young (4 months) or aged (22 months) mice treated with Tri-1 or vehicle control was determined by RT-qPCR. n = 4 biologically independent mice per group. Data represent mean ± s.e.m. P-values were calculated using a two-tailed t test.
