Extended Data Fig. 2: TXNRD1 localizes into CCFs during senescence.
a, Representative images of immunostaining for γH2AX and TXNRD1 in control and oncogenic RAS-induced senescent IMR90 cells. The arrow indicates an example of γH2AX and TXNRD1 positive CCFs in RAS-induced senescent cells. Scale bar = 10 μm. b, Representative images of immunostaining for γH2AX, cGAS and TXNRD1 in young (PD26) and replicative senescent (PD72) IMR90 cells. The arrow indicates an example of γH2AX, cGAS and TXNRD1 positive CCFs in replicative senescent cells. Scale bar = 10 μm. c, Quantification of (a) and (b). d,e, Representative images of immunostaining for γH2AX, cGAS and TXNRD1 in control and oncogenic RAS-induced senescent BJ human fibroblasts. The arrows indicate examples of γH2AX, cGAS and TXNRD1 positive CCFs in RAS-induced senescent cells (d). γH2AX-positive CCFs that are positive for TXNRD1 in senescent BJ cells were quantified (e). Scale bar = 10 μm. Data represent mean ± s.e.m. n = 3 biologically independent experiments.
