Extended Data Fig. 5: snRNA-seq analysis and bulk RNA-seq GSEA of MASLD liver. | Nature Aging

Extended Data Fig. 5: snRNA-seq analysis and bulk RNA-seq GSEA of MASLD liver.

From: Aging promotes metabolic dysfunction-associated steatotic liver disease by inducing ferroptotic stress

Extended Data Fig. 5

(A, C) Uniform manifold approximation and projection (UMAP) visualization of liver cells in single-nucleus RNA-seq dataset generated from two MASLD patients or two healthy controls (GSE174748) (A), or mice fed with CDA-HFD diet or chow diet for 22 weeks (C). (B, D) We focused our analysis on the clusters that are most enriched for hepatocyte markers and depleted for non-hepatocyte markers in both human (B) and mouse dataset (D). GSEA of bulk liver RNA-seq data from Duke MASLD patients (GSE213623) revealed that genes related to (E) apoptosis, (F) pyroptosis and (G) necroptosis are all enriched in the liver transcriptomics of MASLD patients. p values in GSEA plots were calculated using permutation test, then adjusted for multiple comparison testing using the Benjamini-Hochberg method.

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