Extended Data Fig. 6: Livers of MASLD patients exhibit ferroptotic stress.

(A) Duke MASLD cohort patients (GSE213623) with the same fibrosis stage were stratified into young (age ≤ 35), middle age (35 <age < 55) and old groups (age ≥ 55). Expression of ferroptotic-associated genes was compared among the different age groups (age 20-35, fibrosis F0F1, n = 2; F2, n = 9; F3F4, n = 4; age 36-55, fibrosis F0F1, n = 55; F2, n = 61; F3F4, n = 39; age 56-80, fibrosis F0F1, n = 19; F2, n = 37; F3F4, n = 52). Box plot showed the upper quantile (75%), median (50%) and lower quantile (25%) (A) of overall data distribution in MASLD patients with different fibrosis stages. (B) Expression of genes related to iron homeostasis and (C) ferroptotic stress was compared between MASLD patients versus controls (healthy n = 2; MASLD n = 2). The average expression of in each single cell was calculated by the AddModuleScore function of Seurat. Feature plot showed single cells on dimensional reduction plot, with cell color indicating their relative expression levels. Violin plot showed the maxima, upper quantile (75%), median (50%), lower quantile (25%) and minima of overall data distribution in the healthy and MASLD group. p-values were calculated using: Wilcoxon Rank Sum test in A; unpaired two-tailed t.test in B, C.