Extended Data Fig. 7: Ferroptotic stress, lipotoxicity, inflammation and collagen metabolism are increased in livers of old mice.
(A) MDA levels were measured by western blots in livers and primary hepatocytes from chow-fed young (3 months old, n = 4) and old (2 years old, n = 5 for liver lysates, n = 4 for hepatocyte lysates). Data are graphed as mean ± SEM. (B) A published dataset (GSE132042) was re-analyzed to compare the transcriptomes of very old mouse liver ( ≥ 24 months old, n = 7) to young mouse liver ( ≤ 3 months old, n = 12). AHGS was enriched in the transcriptome of liver of aged mice. Boxplot shows the upper quantile (75%), median (50%) and lower quantile (25%) of overall data distribution. GSEA analysis further revealed that the transcriptomes of aged mouse liver are enriched with genes associated with (C) hepatocyte aging, (D) lipid metabolism, (E) inflammation and (F) collagen activity. p-values were calculated using: unpaired two-tailed t.test in A; Wilcoxon Rank Sum test in B; permutation test, then adjusted for multiple comparison testing using the Benjamini-Hochberg method in C, D, E, F.
