Extended Data Fig. 4: Senescent BMMs lead to the propagation of senescent phenotypes to young murine.
a-b, AUC data for GTT and ITT were calculated, respectively (n = 6 mice). c, Western blot analysis of key molecules in the insulin signaling pathway in liver and muscle (n = 3 mice). d, Osteocalcin staining was performed in femoral bone sections after BMMs transplantation (scale bar, 50μm; n = 4 mice; 5 ~ 6 images per mouse). e, Representative images and quantification of the number of PSD95+ synapses that engulfed by microglia (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse) and immunofluorescence detection of GluR-1 in brain (scale bar, 50μm; n = 4 mice; 5 ~ 6 images per mouse). Mean ± SEM are shown for all panels. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.0001 according to one-way ANOVA followed by Tukey’s multiple comparison test.
