Extended Data Fig. 5: Senolytics can eliminate senescent BMMs, and alleviate their pro-aging effects on multiple young tissues.
a-b, Representative images (scale bar, 100μm; n = 5 mice; 5 ~ 6 images per mouse) and quantification of the number of γH2A.X+ macrophage in bone marrow after in vivo DQ treatment. c-d, SA-β-gal staining and immunofluorescence staining of p21, γH2A.X and p53 in BMMs after in vitro DQ treatment (scale bar, 100μm; n = 3 mice; 5 ~ 6 images per n). e, Expression levels of SASP factors in muscle and adipose tissue of young mice transplanted with DQ-treated BMMs (n = 4 mice). f, The AUC data for GTT and ITT were calculated, respectively (n = 6 mice). g, Representative liver Oil-Red staining (scale bar, 50μm; n = 4 mice; 5 ~ 6 images per mouse). h, Quantification of phosphorylation levels of insulin signaling in liver (n = 3 mice for Y-control; n = 4 mice for other groups), muscle (n = 4 mice) and adipose tissue (n = 3 mice). i, Representative images of PSD95 immunofluorescence and Nissl staining in brain (scale bar, 50μm; n = 4 mice; 5 ~ 6 images per mouse). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.0001 were determined using two-tailed t-test in a-d and one-way ANOVA followed by Tukey’s multiple comparison test in e-i.
