Extended Data Fig. 1: Aged bone marrow drives senescence in young recipients.
a, CD45.2 expression in white blood cells (enriched from total blood cells by red blood cell lysis) of CD45.2 recipient mice before irradiation, and CD45.1 and CD45.2 expression in white blood cells after transplantation of CD45.1 donor bone marrow cells (n = 3 mice). b, Expression of senescence-related genes in liver, muscle and adipose tissue of young recipients after bone marrow transplantation (n = 4 mice for ABMT; n = 3 mice for other groups). c, Representative SA-β-gal staining in femoral bone sections (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). d, Representative p53 fluorescence image in brain (scale bar, 50μm; n = 5 mice for ABMT; n = 4 mice for other groups; 5 ~ 6 images per mouse). e, The AUC data for GTT and ITT were calculated, respectively (n = 5 mice). f-g, Western blot analysis of key molecules of insulin pathway in muscle and adipose tissue, and their quantitative data (n = 3 mice). h-i, Representative HE staining and osteocalcin staining in femoral bone sections (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse), and their quantitative data. j-k, Immunofluorescence for GluR-1 protein in brain and quantitative analysis (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). l-m, Nissl staining in brain and quantitative analysis (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.0001, as determined by one-way ANOVA followed by Tukey’s multiple comparison test.
