Fig. 1: GPR158 localized in PC of hippocampal neurons.
From: A primary cilia–autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
a, Volcano plot showing protein fold change between vehicle-treated and OCN-treated hippocampi. b, Protein ontology network showing enrichment for PC core proteins in the hippocampus treated with OCN versus vehicle. c, 2D image (left) and 3D rendering (right) of GPR158 (green) localization in PC from primary hippocampal neurons treated with vehicle or OCN. Scale bar, 1 µm. Graph bar represents the quantification of GPR158 spots in PC (vehicle n = 12, OCN n = 10, from two independent experiments). d, Co-immunoprecipitation of TULP3 with GPR158 or IFT20 in N2a cells and representative image of co-immunofluorescence of GPR158 (green) and TULP3 (white) in the PC stained with ACIII (orange) of hippocampal neurons. Scale bar, 1 µm. e, 2D image (top) and 3D rendering (bottom) of GPR158 (green) localization in PC from Gpr158−/− primary hippocampal neurons infected with WT or mutated Gpr158 and treated or not with OCN. Scale bar, 1 µm. Quantification of GPR158 spots found in each neuronal PC from three independent cultures (in 10–25 neurons per dot). PC was stained by ACIII in each corresponding panel. Data are represented as mean ± s.e.m. The P value was determined by two-tailed Student’s t-test compared to control group (c) or by one-way ANOVA followed by a post hoc Sidak correction (e).
