Extended Data Fig. 3: Reciprocal regulation of the GPR158-OCN coupling system and core PC protein levels.
From: A primary cilia–autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
A-C) Relative gene expression of Ift20, Ift88, Ift25, Kif3a and Tulp3 in the hippocampus of either Ocn-/- (A), Gpr158-/- (B) or WT after stereotactic injections of OCN (C). The quantification is relative to WT (A-B) or WT after vehicle stereotactic injections (C). D-E) Relative gene expression (RT-qPCR) of either Ift20 or Kif3a and representative Western blots of IFT20, KIF3A and GPR158 in 3-month-old mice hippocampi, 3 weeks after hippocampal stereotactic injections with either AAV-U6-shRNA-Ift20 (D), AAV-U6-shRNA-Kif3a (E) or their respective controls. β-actin was used as a loading control for WB analyses. F) LC3B and SQSTM1/p62 immunofluorescence and punctae quantification performed on brain cross sections at the level of the hippocampal Dentate Gyrus (DG) performed in 3-month-old mice, 3 weeks after local stereotactic injections with either AAV-U6-shRNA-Ift20, AAV-U6-shRNA-Kif3a or AAV-U6-shRNA-Scramble. Scale bar: 7 μm. Data are expressed as mean ± SEM. The p-values were determined by a two-tailed Student’s t-test compared to control (vehicle or U6-shRNA-Scrb depending on the experiment).
