Extended Data Fig. 5: Restoration of neuronal IFT20 levels in old hippocampi reverses age-related cognitive deficits, without changing PC size.
From: A primary cilia–autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
A) Primary cilia immunofluorescent staining (ACIII antibody) in the DG region of the hippocampus of from young (3Mo) and aged (16Mo) mice. The length of the primary cilia was measured and quantified in 3D using the Imaris software. Scale bar: 5 μm. B) Primary cilia length quantification obtained after immunofluorescent staining (ACIII) in the CA3 (left panel) and DG (right panel) regions of the hippocampus of 16 month-old mice, 3 weeks after local stereotactic injections with either AAV-Ift20 cDNA (mIft20) or AAV-GFP (Control). The length of the primary cilia was measured and quantified in 3D using the Imaris software. Scale bar: 10 μm. C) Morris water maze test (MWM) performed in 3- and 16-month-old mice, 3 weeks after local stereotactic injections with either AAV-Ift20 cDNA or AAV-GFP (Control). The MWM was performed in 1 cohort of mice. Data are expressed as mean ± SEM. The p values were determined by a two-tailed Student’s t-test compared to 3 Mo in A and by a Two-way ANOVA with multiple comparison in C.
