Extended Data Fig. 6: OCN treatment in old mice improves core PC-protein levels in hippocampus, without reversing PC length.
From: A primary cilia–autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
A) Representative western blot image and quantification of IFT20, KIF3A, and TULP3 in 16- month-old mice 4 h after hippocampal stereotactic injection of either vehicle or OCN. β-actin was used as a loading control for each sample. The quantification is relative to Veh. injected group. B) Primary cilia length quantification obtained after immunofluorescent staining (ACIII antibody) in the CA3 (left panel) region of the hippocampus of 3 and 16- month-old mice after peripheral chronic infusion (peripheral osmotic minipumps) with either vehicle or OCN. The length of the primary cilia was measured and quantified in 3D using the Imaris software. Scale bar: 10 μm. Data are expressed as mean ± SEM. The p values were determined by a two-tailed Student’s t-test compared to veh in A and by a one-way ANOVA with multiple comparison followed by a Tukey test in B.
