Extended Data Fig. 7: OCN enhances CREB phosphorylation and LC3B levels in the CA3 region of hippocampus.
From: A primary cilia–autophagy axis in hippocampal neurons is essential to maintain cognitive resilience
A-B) Phospho-CREB (A) and LC3B (B) immunofluorescence and quantification (relative intensity) performed on brain cross-sections (hippocampal CA3, Dentate Gyrus (DG) and CA1 regions) of 3-month-old mice 4 h after hippocampal stereotactic injection with either vehicle or OCN. Puncta analysis was performed using Icy Software. Scale bar: 50 μm. C) Phospho-CREB immunofluorescence and quantification (relative intensity) performed on brain cross-sections (hippocampal DG region) of 3- and 16-month-old mice, 3 weeks after local stereotactic injections with either AAV-GFP (Control) or AAV-mIft20 cDNA. The analysis was performed in 1 cohort of mice. Scale bar: 20 μm. D) Relative gene expression of c-Fos in the hippocampi of 3-month-old mice previously injected in the hippocampus with either AAV-eSYN-shRNA-Ift20mir or eSYN-shRNA-Scramble-mir. The mice were subjected to stereotactic injection of either vehicle or OCN. Data are expressed as mean ± SEM. The p values were determined by a one-tailed (A, B, D) or a two-tailed (C) Student’s t-test.
