Extended Data Table 4 Treatment effects of vitamin D, omega-3 and SHEP (simple home exercise program) individually and in combination on change of DNAm-based surrogate biomarkers of plasma proteins based on GrimAge

From: Individual and additive effects of vitamin D, omega-3 and exercise on DNA methylation clocks of biological aging in older adults from the DO-HEALTH trial

  1. The seven DNA methylation based estimators of plasma proteins estimate plasminogen activation inhibitor 1 (DNAm PAI-1), beta‐2 microglobulin (DNAm B2M), adrenomedullin (DNAm ADM), leptin (DNAm Leptin), tissue inhibitor metalloproteinase 1 (DNAm TIMP‐1), growth differentiation factor 15 (DNAm GDF‐15), cystatin C (DNAm Cystatin C). For the seven DNAm-based proteins, omega-3 stands out as an individual treatment with a decline in three of seven DNAm-based surrogate biomarkers of plasma proteins (PAI-1, Leptin, TIMP-1). However, the data also shows consistency of an additive benefit combining two or all three treatments for several plasma proteins (PAI-1, B2M, TIMP-1, GDF-15) See Fig. 3 – visual display of the same findings. The table shows regression coefficients, and their 95% CI derived from analysis of covariance adjusted for chronological age (continuous + spline at 85 years), sex, falls before study, body mass index, study site, respective baseline DNAm surrogate marker. All analyses were done in n = 777 participants sampled at baseline and at 3-year follow-up, without technical replicates.
Back to article page