Extended Data Fig. 5: IL-12-dependent status of murine oligodendrocytes and of matured human-oligodendrocyte-like cells. | Nature Aging

Extended Data Fig. 5: IL-12-dependent status of murine oligodendrocytes and of matured human-oligodendrocyte-like cells.

From: Interleukin-12 signaling drives Alzheimer’s disease pathology through disrupting neuronal and oligodendrocyte homeostasis

Extended Data Fig. 5

a. Gene Ontology (GO) of differentially expressed transcripts of different types of oligodendrocytes derived from APPPS1 vs. APPPS1.Il12b−/− mice. Dot size illustrates gene ratio, color denotes P value. One-way ANOVA with Holm Bonferroni P value adjustment. b. Violin plot depicting log2-fold changes in expression of distinct genes of the analysis shown in (a). Red dots indicate genes with positive log2-fold changes, blue dots those with negative log2-fold changes. c. MBP-immunoreactivity in the cortex of APPPS1 and APPPS1.Il12b−/− versus WT control. Scale bar: 100µm. d. Quantification of MBP-covered area, n = 6 biological replicates per genotype, one-way ANOVA showed no significant differences. e. Cartoon illustrating human oligodendroglioma (HOG) cell maturation. f. qPCR of differentiated HOG cell line exemplifying oligodendrocyte-like cells versus non-differentiated HOG cells show a maturation-dependent increase in MBP expression, n = 3 technical replicates, Student’s t-test, P = 0.0178. g. Statistical analysis using two-tailed unpaired students t-test, t = 3.885, df = 4, n = 3 technical replicates. h. Maturation/differentiation-dependent increase in the expression of various pro-inflammatory cytokines in the supernatant of HOG upon stimulation with 85 nM IL-12p70, 85 nM IL-12p80 or vehicle only. Undifferentiated HOG cells are shown in green squares, differentiated HOG cells are in blue squares. All df = 17. F = 19.70 for IL-7, F = 85.55 for TNF-ß, F = 876.8 for VEGFA, F = 155.2 for IL-17, F = 117.4 for IL-1a, F = 232.2 for IL-16, n = 3 technical replicates. i Even doses as low as 1.5 nM IL-12p70 or 1.5 nM IL-12p80 suffice to induce cytokine expression in differentiated HOG cells, while 1.5 nM IL-23 has almost no effect. All df = 11, F = 73.8 for IL-15, F = 12.7 for TNF-ß, F = 67.3 for IL-1a, F = 79.2 for IL-16, F = 10.5 for VEGFA. Analyzed by one-way ANOVA with Tukey’s multiple-comparisons test. Each dot represents one technical replicate (n = 3). Bars represent mean ± s.e.m.

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