Extended Data Fig. 1: Human IL-12/IL-23 receptor expression and mouse snRNA-seq data quality and cluster annotation. | Nature Aging

Extended Data Fig. 1: Human IL-12/IL-23 receptor expression and mouse snRNA-seq data quality and cluster annotation.

From: Interleukin-12 signaling drives Alzheimer’s disease pathology through disrupting neuronal and oligodendrocyte homeostasis

Extended Data Fig. 1

Single-nucleus transcriptome data of 76,533 total nuclei isolated from two postmortem human primary motor cortex derived from portal.brainmap.org 4. a-c. Violin plots showing captured IL12RB1, IL12RB2 and IL23R transcripts per cell type in the human cortex. d,e. Violin plots depicting various murine hippocampal cell types: gene count per cell (d) and UMI count per cell (e), averaging at 1,412 genes and 2,421 UMI, respectively. f-h. Doublet discrimination identified roughly 5% of all nuclei as doublets. From a total of 44 detected clusters, cluster 25, 31, 33, 34, 41, 42, 42 and 44 were flagged as clusters carrying doublets using Scrublet (v.0.21). Clusters with >50% of doublets were removed from further analysis. i. Bar graph depicting biotypes of detected RNA shows that approximately 85.7% of all transcripts were protein-coding followed by 13.4% long non-coding RNAs (lncRNAs). j. Heatmap showing cell type-specific lncRNAs. k. Cellular proportions of our snRNA-seq mouse study reflected the l. published cellular composition of the hippocampus according to the Blue Brain Cell Atlas.

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