Fig. 6: Epigenetic editing at age-hypermethylated CpGs in mesenchymal stromal cells.
a, Scheme of multiplexed epigenetic editing in MSCs at five genomic regions that gain DNAm with aging. b, Scatter-plot of DNAm changes across the three biological replicas. CpGs associated to targeted genes (10-kbp region) are highlighted in the corresponding color. While there is a maximum gain of DNAm at a CpG in PDE4C, the modifications are lower overall and not equal across all CpGs in the target region. c,d, Cumulative distribution functions of DNAm profiles at CpGs that gain or lose DNAm with aging in T cells. Bystanders increase for strata with higher age-relatedness (positive correlation: P < 10−8; NS, negative correlation; Kruskal–Wallis test).
