Extended Data Fig. 4: Analyses of CAFs. | Nature Aging

Extended Data Fig. 4: Analyses of CAFs.

From: Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Extended Data Fig. 4

(a) Dot plot showing expression of all potential ligands predicted by NicheNet for each TME cell type, where dot size and color represent percentage of marker gene expression and the averaged scaled expression value, respectively. (b) Mesenchymal cell distribution of EOPC versus LOPC, with abundance represented by ratio of observed to expected (Ro/e). (c) Correlation plot of BMPs gene expression levels with AR-MP AUCell score in GSE21034. Error bands represented 95% confidence interval (two-sided Spearman’s correlation analysis). (d) Clustering of fibroblasts on the LOPC3 ST slide, related to Fig. 5f. (e) Representative image of multiplex immunofluorescence staining for KLK3 (indicating cancer cells), COL-3 (indicating fibroblasts), FAP (indicating inflammatory CAFs), BMPR1B and BMP4 in 11 EOPC sections from different human PCa tissues.

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