Fig. 1: Aging endometrium exhibits impaired endometrial receptivity.
From: Endometrial aging is accompanied by H3K27ac and PGR loss
a, Schematic design to assess pregnancy outcomes of young and middle-aged patients undergoing PGT-A. b, The impact of age on pregnancy outcomes in patients undergoing PGT-A. c, Representative images showing Ki67 immunohistochemistry (IHC) staining in the human mid-secretory endometrium (n = 3). d, Immunofluorescence (IF) staining of cell morphologies in human endometrial stromal cells during induced decidualization. Decidualization was induced by the treatment of 0.5 μM cAMP and 1 μM MPA. The F-actin cytoskeleton was visualized by rhodamine phalloidin staining. e, Relative mRNA levels of IGFBP1 and PRL in human endometrial stromal cells during induced decidualization (n = 3). f, Representative images illustrating protein levels of IGFBP1 in human endometrial stromal cells during induced decidualization (n = 3). g, Representative images showing PGR IHC staining in the human mid-secretory endometrium (n = 3). h, Representative images showing ERα IHC staining in the human mid-secretory endometrium (n = 3). i, Protein levels of PGR and ERα in the human mid-secretory endometrium (n = 5). j, Relative mRNA levels of PGR and ESR1 in the human mid-secretory endometrium (n = 5). k, FPKM of PGR and ESR1 in the human mid-secretory endometrium (n = 10 and n = 8 for the young and middle-aged groups, respectively). The adjusted P value was determined by DESeq2 (ref. 79). The median, upper and lower quartiles are shown. Whiskers represent upper quartile + 1.5 interquartile range (IQR) and lower quartile − 1.5 IQR. l, Pathway enrichment analysis of downregulated DEGs in the aging mid-secretory endometrium. The adjusted P value was determined by Metascape80. In c, d, g and h, scale bar, 50 μm. The nuclei were stained with hematoxylin in the IHC staining and with DAPI in the IF staining. In e and j, statistical analysis was performed by two-sided unpaired Student’s t-test. Data are presented as mean ± s.d. All replicates were biological replicates. D, day; FPKM, fragments per kilobase of transcript per million mapped reads; ge, glandular epithelium; le, luminal epithelium; M or mid-aged, middle-aged; na, not applicable; ns, not significant; P.adj, adjusted P value; s, stroma; Y, young.
